Contributions of Membrane Components to Intracellular Water Order. The Current Status.
Rept. no. 1 (Annual) 1 Oct 76-30 Sep 77,
WAYNE STATE UNIV DETROIT MICH DEPT OF BIOLOGY
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Methods are described for measuring intracellular viscosity by electron spin resonance and show results obtained for red blood cells, red blood cell ghosts, chloroplasts, and artificial lipid vesicles. The conclusions from these studies are that membrane proteins may be strong contributors to water order within cells and organelles. Detailed descriptions are provided for the methods developed for use in this project. These include preparing a sample for measuring its internal viscosity with the spin label TEMPAMINE 2,2,6,6-tetramethyl piperidine-N-oxyl-4-amine, spin labeling cell and organelle membranes, and preparation of hemoglobin-free resealed red blood cell ghosts.
- Anatomy and Physiology