Accession Number:

ADA045207

Title:

Studies on Metabolism, Function and Mechanism of Destruction of Red Cells.

Descriptive Note:

Annual progress rept. 1 Sep 75-31 Oct 76,

Corporate Author:

ROCHESTER UNIV N Y SCHOOL OF MEDICINE AND DENTISTRY

Report Date:

1976-11-01

Pagination or Media Count:

16.0

Abstract:

The authors have developed a simplified method for measuring oxygen hemgolobin affinity. This method takes advantage of the observation that the oxygen tension of peripheral venous blood is in effect tonometered by the tissues to a value around the P sub 50 O2 for hemoglobin. By using an equation into which the antecubital venous pH, oxygen tension and oxygen saturation is inserted, one can calculate the P sub 50 of the patients hemoglobin. This has proved to be a useful technique for identification of mutant hemoglobins with high or low affinity. Using a dog gracilis muscle model for the study of oxygen transport, they have determined that at normal flow rates, severe alkalosis does not impair either resting or exercising oxygen consumption in spite of the associated reduction in P sub 50 in vivo. During alkalosis, however, the muscle functions at a lower level of venous oxygen tension. Thus, at a reduced arterial oxygen rate, the induced affinity change may have an impact on tissue oxygen uptake.

Subject Categories:

  • Biochemistry
  • Medicine and Medical Research

Distribution Statement:

APPROVED FOR PUBLIC RELEASE