Propagation of Antibody Forming Cells in Diffusion Chambers.
Rept. no. 3 (Final) 1 Mar 73-31 Mar 77,
ILLINOIS UNIV AT URBANA-CHAMPAIGN DEPT OF VETERINARY PATHOLOGY AND HYGIENE
Pagination or Media Count:
Millipore diffusion chambers, implanted into the peritoneal cavity of irradiated recipient mice, were used to culture immunologically competent mouse cells undergoing a secondary response to the hapten DNP. This technique permitted in vitro manipulations to be combined with physiological in vivo maintenance of the cells. Several parameters of the immune response were studied utilizing diffusion chambers, including the maturation of the immune response, genetic control of the magnitude and of the heterogeneity of the immune response and the age-related decline of immune potential. Termination of immunologic tolerance and the induction of tolerance in young-adult and old mice were also studied utilizing in vivo techniques. Author
- Anatomy and Physiology