Accession Number:

ADA043782

Title:

Radioimmunoassay of Urinary Free Cortisol.

Descriptive Note:

Final rept.,

Corporate Author:

NAVAL HEALTH RESEARCH CENTER SAN DIEGO CALIF

Report Date:

1976-09-28

Pagination or Media Count:

10.0

Abstract:

The radioimmunoassay for urinary free cortisol described in this paper is simple, rapid, and reproducible. The method uses a commercially available antibody preparation and is performed in two steps. The first step includes an extraction and a column purification to remove materials antigenically similar to cortisol from the urine. The second step is the radioimmunoassay using dextran coated charcoal to separate bound and unbound cortisol. H3-cortisol is added prior to any mechanical manipulation to allow calculation of analytical recovery for the purification procedure. The coefficient of variation for interassay determinations was a maximum of 10.3 and for intra-assay determinations a maximum of 5.7. Analytical recovery averaged 97.7. One technician can analyze 100 samples per week. Author

Subject Categories:

  • Biochemistry

Distribution Statement:

APPROVED FOR PUBLIC RELEASE