Immunological Characterization of Type-Specific and Group-Reactive Dengue Virus Soluble Antigens.
Annual progress rept. Jul 73-Jul 74,
TEXAS UNIV HEALTH SCIENCE CENTER AT SAN ANTONIO
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A micro-solid-phase radioimmunoassay SPRIA is described for quantitation of antibodies to purified flaviviruses as well as to the purified envelope glycoprotein and 80,000-molecular-weight viral nonstructural protein. Sera from mice experimentally infected with Saint Louis encephalitis SLE virus or from humans after a primary SLE virus infection reacted more specifically with the major viral envelope protein in the SPRIA test than with antigens conventionally used in the complement fixation CF and hemagglutination inhibition tests. A high degree of correlation P 0.05 was observed between SPRIA antiimmunoglobulin G binding values with the 80,000-molecular-weight nonstructural protein of SLE virus and antibody titers obtained by plaque reduction neutralization and CF with the nonstructural protein.