Accession Number:

ADA043445

Title:

Differential Protection of Mammalian Cells from Pseudomonas and Diphtheria Exotoxins by Exogenous Nucleotides,

Descriptive Note:

Corporate Author:

ARMY MEDICAL RESEARCH INST OF INFECTIOUS DISEASES FREDERICK MD

Report Date:

1977-01-01

Pagination or Media Count:

33.0

Abstract:

Exogenous nucleotides were found to protect mammalian cells from Pseudomonas PE and diphtheria DE exotoxins. The protection was dose-dependent and required the simultaneous presence of nucleotide during toxin challenge. Protection from DE was proportional to the number of phosphates in the nucleotides, exhibited base specificity, and required an intact nucleotide for full expression. Protection from PE seemed to require only phosphate oligomers. Neither phosphorylation nor interference with the intracellular enzymatic activities of the toxins appeared to mediate the protection. It was concluded that exogenous nucleotides block the attachment or internalization stages of intoxication. Author

Subject Categories:

  • Biochemistry
  • Pharmacology

Distribution Statement:

APPROVED FOR PUBLIC RELEASE