Effects of Beta-Aminoethylisothiourea (AET) on Enzymes of Animal Tissues
SCHOOL OF AVIATION MEDICINE RANDOLPH AFB TX
Pagination or Media Count:
The effects of the radioprotective compound beta- aminoethylisothiourea AET on sulfhydryl enzymes of mammalian tissues have been determined, in an effort to obtain information regarding the biologic activity of the drug. A number of enzyme systems involved in oxidative carbohydrate metabolism of rat tissues were inhibited in vitro by concentrations of AET within the predicted in vivo range following sublethal doses. Two of these enzyme systems, the pyruvic and alpha-ketoglutaric acid oxidizing systems of rat liver and kidney, were also markedly inhibited in vivo by AET. Inhibition was not due to an effect on coenzymes involved in the reactions but may have been caused by inactivation of the electron transport system. The results of this study demonstrate the feasibility of a theory of radioprotective mechanisms involving the formation of radioresistant complexes between the protective agent and important biologic sulfhydryl groups.