A Method for Studying Changes in Profibrinolysin Titer in Dogs Following in Vivo Induction of Fibrinolytic Activity
SCHOOL OF AVIATION MEDICINE RANDOLPH AFB TX
Pagination or Media Count:
A two-stage assay for canine profibrinolysin plasminogen is described. This assay is suitable for following in vivo changes in profibrinolysin titer induced by various experimental procedures. Plasma profibrinolysin is concentrated by a low ionic strength, protamine sulfate precipitation technic and activated with staphylokinase. Diluted aliquots of the activated solution are then assayed for fibrinolytic activity. One unit is that amount of activity which at 37 degrees C. and pH 7.25 will bring about lysis of 0.4 ml. of a 0.1 percent bovine fibrin clot in 300 seconds. The average dog plasma contains 19.4 units per milliliter. In general, increases in circulating fibrinolytic activity are associated with decreases in profibrinolysin.