Modifications of the Reddy Method for the Estimation of 17-Hydroxycorticoids in Blood
SCHOOL OF AVIATION MEDICINE RANDOLPH AFB TX
Pagination or Media Count:
A procedure evolved from modifications of existing technics for the estimation of total 17-hydroxycorticoids in blood has been described. The precision of the method based on the average difference between 95 sets of duplicates indicated a standard deviation of 5.48 and a coefficient of variation of 10.38 percent. Essentially, quantitative recoveries mean, 98 percent were obtained by adding hydrocortisone to protein-free plasma filtrates relatively poor recoveries mean, 65 percent resulted from addition of hydrocortisone to untreated plasma samples. A range of 9 to 82 gamma percent was found in a series of 52 subjects, with a mean of 41.2 gamma percent.