Accession Number:

ADA036017

Title:

Purification Techniques for Living Rickettsiae,

Descriptive Note:

Corporate Author:

ARMY MEDICAL RESEARCH INST OF INFECTIOUS DISEASES FREDERICK MD

Personal Author(s):

Report Date:

1977-02-22

Pagination or Media Count:

11.0

Abstract:

Numerous techniques for the purification of Rickettsiae exist at the present moment. Their high number indicates the extent of the problem. To obtain purified Rickettsiae which are still viable, techniques which involve simple centrifugation in the presence of KCl Mi or, even better, those in which either Amberlit or Sephadex is added to the centrifuged preparation, are recommended. These methods are only slighty more complicated than the technique of Craigie and have the advantage of yielding living suspensions in which the bacterial coat is only slightly altered and whose virulence is only slightly reduced. Author

Subject Categories:

  • Microbiology
  • Pharmacology

Distribution Statement:

APPROVED FOR PUBLIC RELEASE