Contribution of Circulating Blood Cells to Survival of Mice Experiencing Endotoxemia
ARMED FORCES RADIOBIOLOGY RESEARCH INST BETHESDA MD
Pagination or Media Count:
The hypothesis that platelets and granulocytes play a significant role in mediating the lethal consequences of endotoxemia associated with gram-negative infections was evaluated by studying responses to endotoxin challenge in mice made leukopenic and thrombocytopenic by irradiation. Sensitivity to endotoxin entering the circulation from the intestine was increased in animals deficient in granulocytes and platelets. Alterations in blood enzyme levels measure during endotoxemia were different in unirradiated and irradiated mice. The administration of 0.4 mg ZnCl2 or 5 mg cortisone acetate prior to challenge with a lethal dose of Salmonella typhosa endotoxin provided significant protection against the toxin in unirradiated mice, where as only cortisone protected the irradiated animals. Zinc protection against endotoxin challenge in unirradiated mice correlated with high blood levels of the ion. Administration of zinc increases the number of circulating leukocytes available at the time of endotoxin challenge, but cortisone promotes a later recovery of leukocyte numbers. Plasma glucose was elevated in zinc-treated mice.
- Medicine and Medical Research