Serological Diagnosis of Gonorrhea.
Final rept. 1 Sep 73-30 Nov 76,
MONTANA UNIV MISSOULA DEPT OF MICROBIOLOGY
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An antigen specific for Neisseria gonorrhoeae was extracted with a surfactant from T-1 phase cells of strain F-62 this antigen was purified partially by fractional ethanolic precipitation and centrifugation. The anitgen was characterized as a low molecular weight glycoprotein. With a c14-labeled preparation of this gonococcal antigen, a radioimmunoassay was developed the serodiagnosis of gonorrhea. When 382 human sera from acutely infected and well documented negative control subjects were assayed by this procedure, 90 were diagnosed correctly, 5 were diagnosed incorrectly, and 5 of the results were indecisive. The assay procedure was modified in that the antigen was labeled with a more easily quantifiable isotope, 125I. In this latter system, 77 of the sera were diagnosed correctly, 13 were false positive or negative, and 10 of the results were indecisive. Author
- Medicine and Medical Research