Accession Number:

ADA034281

Title:

Serological Diagnosis of Gonorrhea.

Descriptive Note:

Final rept. 1 Sep 73-30 Nov 76,

Corporate Author:

MONTANA UNIV MISSOULA DEPT OF MICROBIOLOGY

Personal Author(s):

Report Date:

1976-12-01

Pagination or Media Count:

10.0

Abstract:

An antigen specific for Neisseria gonorrhoeae was extracted with a surfactant from T-1 phase cells of strain F-62 this antigen was purified partially by fractional ethanolic precipitation and centrifugation. The anitgen was characterized as a low molecular weight glycoprotein. With a c14-labeled preparation of this gonococcal antigen, a radioimmunoassay was developed the serodiagnosis of gonorrhea. When 382 human sera from acutely infected and well documented negative control subjects were assayed by this procedure, 90 were diagnosed correctly, 5 were diagnosed incorrectly, and 5 of the results were indecisive. The assay procedure was modified in that the antigen was labeled with a more easily quantifiable isotope, 125I. In this latter system, 77 of the sera were diagnosed correctly, 13 were false positive or negative, and 10 of the results were indecisive. Author

Subject Categories:

  • Medicine and Medical Research

Distribution Statement:

APPROVED FOR PUBLIC RELEASE