Receptor Interaction between Eastern Equine Encephalitis Virus and Chick Embryo Fibroblasts.
ARMY MEDICAL RESEARCH INST OF INFECTIOUS DISEASES FREDERICK MD
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The attachment of eastern equine encephalitis virus to chick embryo fibroblasts was studied at 0 C. The binding specifically responsible for initiating infection was studied in the initial experiments by using plaque forming ability as the measured response. Results from these initial studies were closely paralleled in studies of binding of radiolabeled virus under the same conditions. Binding that had occurred at the pH optimum, pH 6.5, could be reversed only at higher pH. The observed pH dependence of virus attachment suggested the interaction of at least 2 ionizable species in the initial binding of virus to cell and that 1-3 attachments must occur between virus and cell prior to infection. Author
- Medicine and Medical Research