Separation of Long and Short Chain Fatty Acids as Naphthacyl and Substituted Phenacyl Esters by High Performance Liquid Chromatography.
Rept. for Sep 75-Aug 76,
ARMY INST OF DENTAL RESEARCH WASHINGTON D C
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High performance liquid chromatography of various C2 - C24 fatty acids was run on their p-bromophenacyl, p-nitrophenacyl, p-chlorophenacyl, and 2--naphthacyl esters. All the separations were accomplished using reversed phase columns with the eluent consisting of an acetonitrilewater gradient. For all derivatives tested the separations were well defined and analogous although certain esters eluted together as one peak. Quantitative results indicate that the limit of detection in the present study was two picograms of n-caproic acid and 10 picograms of arachidonic acid as their p-bromophenacyl esters.
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