A Study of the Factors Affecting the Cultivation of Malaria Parasites 'in vitro'.
Final technical rept. 1 Dec 75-1 Mar 76,
NATIONAL INST FOR MEDICAL RESEARCH LONDON (ENGLAND) DIV OF PARASITOLOGY
Pagination or Media Count:
There have been many attempts to grow the erythrocytic stages of Plasmodium knowlesi in vitro and although the parasite will develop relatively easily through one asexual cycle it cannot yet be subcultured continuously through several generations. This progressive inability of the parasite to enter and grow in host erythrocytes has been attributed in part to the metabolic defects developing in the parasite during the first asexual cycle in vitro and which become more pronounced with subculture. Uninfected erythrocytes are known to develop metabolic abnormalities when incubated in vitro at 37C which could affect the long term cultivation of the parasite. Some of these changes which occur when uninfected rhesus monkey erythrocytes are incubated in vitro are described and discussed in relation to the susceptibility of these cells to invasion by P. Knowlesi.