Procedures for Determination of Norepinephrine, Epinephrine, and Dopamine in Biological Specimens.
Medical research progress rept.,
NAVAL MEDICAL RESEARCH INST BETHESDA MD
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Methods for catecholamine determination in human blood and urine have been adapted for use. Free norepinephrine NE in plasma was determined by trihydroxyindole THI fluorometric method recoveries ranged from 71-92, mean 82. Duplicate samples yielded results with standard deviation S.D. of or - 37, while replicate determinations performed on the same day varied by or - 10. The mean normal concentration of NE in human plasma N 14 was found to be 2.4 or - 0.9 ngml. Concentrations of NE, epinephrine E and dopamine D were differentially determined from a uniform urine specimen by a similar THI procedure. Recoveries of NE and E ranged from 73-110, mean 85 or - 16. Day-to-day reproducibilities were or - 16 NE and or - 10 E. Replicate samples varied by no more than 10. Dopamine recoveries ranged from 85-108, with a reproducibility of or - 8. Normal ranges for 24-hour excretion N 50 were found to be 0-30 micrograms E mean 13, 10-80 micrograms NE mean 54, and 100-400 micrograms dopamine mean 224. Author