Prostaglandin El-Morphine Effects on Adenyl Cyclase Activity in a Rat Brain Slice System.
ARMED FORCES RADIOBIOLOGY RESEARCH INST BETHESDA MD
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Prostaglandin E1 stimulated adenyl cyclase was studied in a rat brain slice incubation system to determine if stimulation could be reversed by morphine. Brain slices were prepared from naive, acutely morphine intoxicated, and tolerant and withdrawing rats. Morphine addition to prostaglandin E1 stimulated slices did not prevent or reverse adenyl cyclase stimulation in any of the systems. The data do not support the hypothesis that prostaglandin E1 sensitive adenyl cyclase mediates direct biochemical effects of morphine. The prostaglandin E1 stimulated adenyl cyclase system described above serves as a neurochemical model system which may be used to detect and study insults to the central nervous system induced by a variety of toxic agents including ionizing and nonionizing microwave radiation.