Studies on Gonococcus Infection.
Final comprehensive rept.,
UTAH UNIV SALT LAKE CITY COLL OF MEDICINE
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The purpose of work carried out under the auspices of this contract has been the study of gonococcal interactions with eukaryotic cells. The results that were obtained are summarized below 1 Pili enhance attachment of gonococci to tissue culture cells in vitro. 2 Pili enhance attachment of gonococci to human sperm, in vitro. 3 Interactions between gonococci and peripheral blood leukocytes are modulated, primarily, by a surface component of gonococci that is distinct from pili and has been called leukocyte association factor. Gonococci are killed by leukocytes after this initial interaction. 4 Presence or absence of leukocyte association factor plays no role in the interactions between gonococci and tissue culture cells. 5 Gonococci can be easily labelled with 125 Iodine by the lactoperoxidase-hydrogen peroxide method. 6 Leukocyte association factor does not influence the virulence of gonococci as assessed in a subcutaneous model in the mouse or in the 11 day old chick embryo model. 7 Pili reduce the interactions between gonococci and mouse peritoneal macrophages, in vitro. This interaction is not influenced by leukocyte association factor.