Effects of Chemotherapy on Astrocyte Density in the Rat Spinal Cord Dorsal Funiculi after Stab-wound Lesion: I. 5-Fluorouracil. II. Alphamethyl-tyrosine. III. Dexamethasone. IV. Simultaneous dexamethasone and 5-Fluorouracil.
Rept. no. 3 (Final), 1 Jun 72-31 Mar 76,
NORTHERN ARIZONA UNIV FLAGSTAFF DEPT OF BIOLOGICAL SCIENCE
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Rats were lesioned by stab wound in the mid-thoracic spinal cord. Chemotherapy was used in attempt to reduce the post-lesion density of astrocytes. Four experiments were conducted, each with a different chemotherapy regimen 5-fluorouracil 5-FU, alpha methyl-tyrosine AMT, dexamethasone, and dexamethasone and 5-FU together. Astrocyte densities were determined by light microscopy. The 5-FU treated cords lead to an apparent 13.1 decrease in astrocyte density this density is not significantly different from the control cords. AMT therapy resulted in an average astrocyte density that is 11.1 greater than the control lesion and no chemotherapy. Dexamethasone treatment appeared to have increased astrocyte density by 22 but this had questionable statistical significance. Simultaneous dexamethasone and 5-FU chemotherapy may have produced a small cell density increase. There is no convincing evidence that use of these compounds will reduce the post-lesion glial scar.
- Medicine and Medical Research