Serum Protein-Bound Hexosamine Analysis with Autoclave Hydrolysis.
ARMED FORCES RADIOBIOLOGY RESEARCH INST BETHESDA MD
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A modification of the Winzler method for assay of serum protein-bound hexosamine is presented. The modified method eliminates the usual 4-hour hydrolysis period by substituting a 15-minute hydrolysis in an autoclave, thus allowing a more rapid processing of samples. No significant differences were found between assay values obtained with either method for a variety of samples. In addition, the concentration of the samples may be effectively doubled and the curve moved to a more sensitive area of the spectrum without loss of sensitivity or accuracy.