Accession Number:

ADA025272

Title:

Serum Protein-Bound Hexosamine Analysis with Autoclave Hydrolysis.

Descriptive Note:

Technical note,

Corporate Author:

ARMED FORCES RADIOBIOLOGY RESEARCH INST BETHESDA MD

Personal Author(s):

Report Date:

1976-03-01

Pagination or Media Count:

16.0

Abstract:

A modification of the Winzler method for assay of serum protein-bound hexosamine is presented. The modified method eliminates the usual 4-hour hydrolysis period by substituting a 15-minute hydrolysis in an autoclave, thus allowing a more rapid processing of samples. No significant differences were found between assay values obtained with either method for a variety of samples. In addition, the concentration of the samples may be effectively doubled and the curve moved to a more sensitive area of the spectrum without loss of sensitivity or accuracy.

Subject Categories:

  • Biochemistry

Distribution Statement:

APPROVED FOR PUBLIC RELEASE