Antibody Formation and Release in Ileal Loops Exposed to Enterotoxin.
Annual rept. 1 Jul 74-31 Dec 75,
JOHNS HOPKINS UNIV BALTIMORE MD
Pagination or Media Count:
The investigation has contained two parts a Study of antibody formation to cholera toxin CT by intestine. b Study of IgA release from crypt epithelium following stimulation by CT. Formation of antibody to CT was accomplished using Thiry-Vella T-V loops in rabbits. It was shown that IgA anti-CT formation was greatest with local intraloop immunization with CT, least with subcutaneous immunization, and intermediate when the two routes were combined. The latter effect suggested suppression of local immunization by systemic immunization under the conditions of the experiment. Protection by local immunity against the vascular permeability and diarrheagenic effects of CT were also demonstrated. IgA release by CT was studied by using immunofluorescent staining for IgA in serial biopsies from acutely prepared T-V loops in non-immunized animals. Crypt cell IgA was thereby demonstrated to be significantly more reduced in loops exposed to CT then in saline-treated control loops by 180 min. The loss of IgA from crypts tended to follow fluid secretion from the loops.
- Medicine and Medical Research