Studies on Typhus and Spotted Fever.
Annual progress rept. 1 Jul 74-30 Jun 75,
MARYLAND UNIV BALTIMORE DEPT OF MICROBIOLOGY
Pagination or Media Count:
Contents Competitive inhibition by cell membrane fragments of rickettsial uptake by CE cells in suspension Estimation of absolute number of infectious rickettsiae Further characterization of growth cycle of Rickettsia prowazeki in CE cell cultures Growth of R. prowazeki in enucleated cells Temperature sensitivity of uptake, growth, viability and plaque formation by R. prowazeki Demonstration of an antigenic slime layer on virulent and attenuated R. prowazeki Production of suspensions of purified intact R. prowazeki spheroplasts Lysis of purified spheroplasts under controlled conditions and visualization of genome Antibiotic resistant mutants of R. prowazeki Dual infection of CE cells with two strains of R. prowazeki and selective intracellular lysis of one strain through formation of penicillin-induced unstable spheroplasts Production of plaque-purified seeds of spotted fever group rickettsiae Studies on the plaques of spotted fever group rickettsiae In vitro infection cycle of spotted fever group rickettsiae Ultrastructure of Rickettsia rickettsii Serological differentiation of field isolates of spotted fever group Selected references.