Excitability and Chemosensitivity Properties of a Somatic Cell Hybrid between Mouse Neuroblastoma and Sympathetic Ganglion Cells.
ARMED FORCES RADIOBIOLOGY RESEARCH INST BETHESDA MD
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A somatic cell hybrid line, NX31, formed by fusion of mouse neuroblastoma and mouse sympathetic ganglion cells has been studied for its electrophysiologic properties and its chemosensitivity to iontophoretically applied acetylcholine ACh. The hybrid cell line was electrically excitable, and its excitability characteristics were found to be closer to those of its putative neuronal parent than to those of its neuroblastoma parent. Fifty percent of the hybrid cells were sensitive to ACh. Two types of depolarizing responses were evoked an early, rapidly developing response and, less frequently, a late, slowly developing response. Neuroblastoma cells may exhibit only the early, rapid response sympathetic neurons show depolarizations corresponding to both types of responses. These findings suggest that the phenotypes expressed by hybrid cells more closely resemble those of sympathetic neurons than do those of their neuroblastoma parents. The possibility is raised of further exploiting the strategy of hybridizing a dividing cell line with other types of neurons to obtain new neuronal cell lines.
- Medicine and Medical Research