Cryotolerance of Selected Sites on the Surfaces of Membranes of Human Red Blood Cells and Leukocytes.
Final technical rept.,
MEDICAL COLL OF WISCONSIN MILWAUKEE DEPT OF PATHOLOGY
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The research is concerned with the development of methodologies for the drying of biologic materials containing dimethylsulfoxide, by sublimation of ice in vacuo. The authors have studied the effects of cyclic freezing and thawing, rates of cooling and storage for 30 to 60 days at different temperatures on the Rh sites on the surface of human red blood cells using the Coombs test. The results of these tests show no demonstrable changes in the ability of the Rh sites to bind antibody following cyclic freezing and thawing, different rates of cooling or storage at low temperatures for 30 or 60 days. The use of an adsorption and agglutination technique to study antigenic determinants on the red cell membrane has been used to detect changes in these sites. It appears that the effects of cryobiologic applications on these surface structures may be less severe than can be determined by adsorption agglutination procedures. The above is particularly true in the Coombs technique.
- Anatomy and Physiology
- Medicine and Medical Research