Freeze-Drying of Solutions of Serum Albumin Containing Dimethylsulfoxide,
MEDICAL COLL OF WISCONSIN MILWAUKEE DEPT OF PATHOLOGY
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Although several publications have emphasized the inadvisability of drying biologic materials containing dimethylsulfoxide DMSO by sublimation of ice in vacuo, the current studies showed a relationship to exist between the relative concentrations of serum albumin, human, and dimethylsulfoxide for successful or unsuccessful freeze-drying of albumin-DMSO solutions. The cycle of freeze-drying for the successful drying of an albumin-DMSO solution was a modification of the cycle used for the successful drying of suspensions of measles virus by sublimation of ice in vacuo. Using nuclear magnetic resonance spectroscopy, a strong, sharp signal for DMSO was obtained in preparations of freeze-dried albumin-DMSO solutions rehydrated with deuterium oxide, D2O.