Accession Number:

ADA023290

Title:

Freeze-Drying of Solutions of Serum Albumin Containing Dimethylsulfoxide,

Descriptive Note:

Corporate Author:

MEDICAL COLL OF WISCONSIN MILWAUKEE DEPT OF PATHOLOGY

Report Date:

1975-05-19

Pagination or Media Count:

19.0

Abstract:

Although several publications have emphasized the inadvisability of drying biologic materials containing dimethylsulfoxide DMSO by sublimation of ice in vacuo, the current studies showed a relationship to exist between the relative concentrations of serum albumin, human, and dimethylsulfoxide for successful or unsuccessful freeze-drying of albumin-DMSO solutions. The cycle of freeze-drying for the successful drying of an albumin-DMSO solution was a modification of the cycle used for the successful drying of suspensions of measles virus by sublimation of ice in vacuo. Using nuclear magnetic resonance spectroscopy, a strong, sharp signal for DMSO was obtained in preparations of freeze-dried albumin-DMSO solutions rehydrated with deuterium oxide, D2O.

Subject Categories:

  • Biochemistry

Distribution Statement:

APPROVED FOR PUBLIC RELEASE