Biochemical Aspects of Alcohol Tolerance and Dependence and Biochemical Aspects of Morphine Tolerance and Physical Dependence.
Final rept. 28 Mar 72-28 Feb 76
MICHIGAN STATE UNIV EAST LANSING DEPT OF PHARMACOLOGY
Pagination or Media Count:
The effects of morphine on protein synthesis were studied in an effort to determine whether altered protein synthesis is involved in the development of tolerance to or physical dependence on morphine. Although alterations in protein synthesis were observed, it appeared that such alterations are the consequences of morphine dependence and withdrawal, rather than a cause of these phenomena. The specific binding of opiates and their congeners to the brain tissue was studied in rats. Characteristics of the specific binding and the conditions for the binding studies which may be relevant to the drug-receptor interaction in vivo were elucidated. The effects of age on the development of tolerance and physical dependence produced by morphine treatment were studied. Although there were no remarkable differences in the development of physical dependence to morphine due to the difference in age of rats, the development of tolerance to morphine was clearly influenced by the age of the subject. The relationship between the development of tolerance to ethanol and alterations in brain Na,K-ATPase was studied. It was concluded that the Na,K-ATPase is not involved in the development of central tolerance to ethanol in rats. It appears that ethanol favors the fluid or less-ordered state of membrane lipids.