Replica Techniques for Transmission Electron Microscopy.
NAVAL RESEARCH LAB WASHINGTON D C
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This report concentrates soley on the description of variations in replicating procedures for the transmission electron microscope. It includes techniques for cellulose acetate and direct carbon replication for rough and flat fracture surfaces, collodion dry stripped replicas of polished and etched or flat fracture surfaces, preparation of substrate films on grids for particle examination, and variations in replica wash techniques i.e., fishing reflux unit screen-bridge and also wax techniques for replicas which tend to fragment badly. The appendix includes material on minimization of artifacts produced during replica preparation, salvage of scrolled replicas, edge retention during replication, and stereo effects. Throughout the text, variations in technique are discussed in order to guide the reader in the selection of an appropriate method to prepare replicas of a given structure. A variety of specialized procedures and detailed explanations are included as well as suggested replicating utensils for basic and specialized techniques. Author
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