Non-Thermal Hazards of Exposures to Radio Frequencies Fields. Microwave studies.
Final rept. 1 Mar 69-30 Nov 74,
NEW ENGLAND INST RIDGEFIELD CONN
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The studies reported include cytogenetic and genetic tests in vitro and in vivo. Human lymphocyte cultures and Chinese hamsters and Drosophila tissue cultures of lung, bone narrow, lymphocytes, germ cells, corneal epithelium, hair follicle cells and brain cells were studied. The hamsters and Drosophila were irradiated in vivo as well. These studies were undertaken to determine effects of radio frequencies 15-40 MHz and microwaves X and K band on the integrity of the genetic system. Radio frequencies in the range of 15-40 MHz were significantly effective in inhibiting mitosis and in producing chromosome abnormalities in human lymphocytes and Chinese hamster lung cells in culture. Significant increases in chromosome abnormalities occurred following irradiation at 21, 25 and 40 MHz. In vivo treatment of Chinese hamster bone marrow and testicular cells at 20, 25, 30 and 35 MHz for 1 to 9 days induced significant increases of aneuploid and polyploid cells, but very few chromosome breaks. Corneal epithelium hair follicle cells were negative. Brain cells of Drosophila larvae also showed no evidence of nondisjunction at these frequencies.