Accession Number:

ADA018552

Title:

Composition and Function of Phospholipids in Plasmodium Knowlesi.

Descriptive Note:

Annual technical rept. 1 Oct 74-31 Oct 75,

Corporate Author:

MARYLAND UNIV COLLEGE PARK DEPT OF CHEMISTRY

Personal Author(s):

Report Date:

1975-11-01

Pagination or Media Count:

48.0

Abstract:

The lipid extracts of normal and Plasmodium knowlesi-infected rhesus erythrocytes and of the parasite itself have been examined for phospholipid composition on an animal-to-animal basis. Phosphatidylinositol PI, phosphatidylcholine PC, and phosphatidylethanolamine PE represented larger percentages in the parasite than in the host. Sphingomyelin SM and phosphatidylserine PS averaged less than 20 and 33 respectively in the parasite than in the infected erythrocyte. The phospholipid distribution and the ratio of cholesterol-to-cholesterol ester were determined for plasma isolated from normal, fasted, and Plasmodium knowlesi-infected monkeys. Compared to normal plasmas, the infected plasmas showed patterns with slight increases in PC and large decreases in lysophosphatidylcholine LPC. The cholesterol-to-cholesterol ester ratio was always less than one in normal and greater than one in infected plasmas. Fasting produced no significant differences on normal samples. A successful high-pressure liquid chromatography separation of LPC, PE, PI, PS, SM, and phosphatidic acid has been achieved with synthetic samples.

Subject Categories:

  • Biochemistry
  • Medicine and Medical Research

Distribution Statement:

APPROVED FOR PUBLIC RELEASE