Freeze Preservation of Red Blood Cells with Hydroxyethyl Starch as a Cryoprotective Agent.
VETERANS ADMINISTRATION HOSPITAL ANN ARBOR MICH
Pagination or Media Count:
Pre-freeze washing increases the saline stability of red cells frozen with 14 hydroxyethyl starch 30 ml units. However, the presence of plasma in the freezing mixture does not improve the ability of the cells to survive a freeze-thaw cycle. This would allow washing before freezing to remove plasma proteins, leucocytes, etc. Large units 385-400 ml frozen with 14 HES yield cell recoveries of 97, saline stabilities of 82-84 and levels of supernatant hemoglobin near 550 mg. ATP and 2-3 DPG levels drop approximately 30 and 20 respectively during freeze-thaw and potassium is lost from the cells. Storage of the thawed units at 4C for as long as 48 hours results in little change in the above parameters. Post-thaw washing causes increased damage to the cells when dilution of the freezing mixtures occurs.
- Anatomy and Physiology