Specificity and Immunosuppressive Potency of a Rabbit Antimouse T Cell- Specific Antiserum
Medical research progress rept.
NAVAL MEDICAL RESEARCH INST BETHESDA MD
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An attempt was made to prepare a specific heterologous rabbit antimouse T cell antiserum anti-MTLA by absorbing rabbit antimouse thymocyte globulin ATG with spleen cells from BALBc T sub X B sub M mice. Cytotoxicity data showed that whereas ATG was cytotoxic to both T and B cells, anti-MTLA was highly cytotoxic to only T cells. Whereas spleen cells treated with ATG and complement C failed to respond in all assays studied, spleen cells treated with anti-MTLA and C 1 responded to the B cell mitogens but failed to respond to the T cell mitogens 2 were able to stimulate allogeneic spleen cells but failed to respond in mixed lymphocyte culture 3 failed to act as T killer cells in the CML reaction but retained their ability to kill antibody- coated target cells and 4 did not cause graft-versus-host reaction when injected in allogeneic mice and increased their survival significantly. Furthermore, anti-MTLA was just as immunosuppressive in vivo as ATG in its ability to suppress the immune response to sheep red blood cells and prolong skin allograft survival. Anti-MTLA was found to be different in specificaties from anti-theta serum by several points. These data indicate that anti-MTLA is a specific antiserum against a unique marker on T cells distinct from the theta marker.
- Anatomy and Physiology