Self Diffusion in Cells and Tissues.
Rept. no. 1 (Annual), 15 Nov 73-30 Jun 75,
NAVAL WEAPONS SUPPORT CENTER CRANE IND
Pagination or Media Count:
The primary objective of this project is to obtain as much information about the state and mobility of water in living cells as can be obtained by nuclear magnetic resonance NMR. Intracellular coefficients of self diffusion of water, and permeabilities of the cell membranes to water are to be measured using pulsed-field-gradient, spin-echo NMR. By the use of this method, the effect of diffusion boundaries with a spacing of the order of colloidal dimensions may be resolved and the true mobilities of substances within the boundaries may be determined. In addition to determining diffusion coefficients representing an average mobility of the water throughout the cell, it may be possible to resolve the fine structures representing intracellular barriers to diffusion or representing regions where the diffusion coefficient is significantly different from the rest of the cell.
- Anatomy and Physiology