Effects of Oxygen and Reduced Glutathione on the Oxygen Consumption of Mouse Liver.
ARMY AEROMEDICAL RESEARCH LAB FORT RUCKER ALA
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The effects of hyperbaric oxygen tensions on the oxygen consumption of mouse liver homogenates was investigated. Hyperbaric oxygen rapidly inhibits the oxidative metabolism of the mammalian liver. Mouse liver homogenate exposed to a PO2 of 3837.8 mm Hg for 30 minutes showed a 50.6 reduction in oxygen consumption compared to controls exposed to nitrogen at ambient pressure. The effect of reduced glutathione GSH as a protective agent against hyperbaric oxygen toxicity was also examined. Liver homogenates pretreated with GSH and exposed to high oxygen tensions demonstrated greater activity than untreated controls. It is concluded that 1 GSH protects important enzymes of oxidative metabolism by keeping them in a reduced and viable state, and 2 GSH can stimulate oxygen consumption by increasing succinate formation through a GSH-succinate shunt.
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