Inhibition of Cellulases of Wood-Decay Fungi.
Forest Service Research paper,
FOREST PRODUCTS LAB MADISON WIS
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The effects of classic enzyme inhibitors, phenols, and extracts from wood on activity of cellulases from two brown-rot and two white-rot fungi were investigated. The cellulases from the fungi varied in their tolerances to the inhibitors. This suggested structural differences in the enzymes. C sub x cellulases, which break down soluble cellulose, were not inhibited in any of the fungi by sulfhydryl-binding compounds and metal-complexing agents. Thus, sulfhydryl groups apparently are not an active site of C sub x, and the enzyme does not require metal. Most phenols inhibited cellulase only when oxidized, and generally inhibited C sub x of the white-rot fungi more than C sub x of the brown-rot fungi. The purpose of the study is to investigate the inhibition of cellulases from wood-decaying fungi by using the following classical enzyme inhibitors, phenolics, and extracts from wood.
- Wood, Paper and Related Forestry Products