Exposure of Dog Erythrocytes In Vivo to Phenylhydrazine and Monomethylhydrazine: A Freeze-Etch Study of Erythrocyte Damage
AIR FORCE AEROSPACE MEDICAL RESEARCH LAB WRIGHT-PATTERSON AFB OH
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Dog red blood cells were exposed in vivo to phenylhydrazine or monomethylhydrazine by injecting the dogs subcutaneously with these compounds. The cell damage was evidenced clinically by hemolytic anemia at all concentrations in the phenylhydrazine exposed animals. In contrast, there was little clinical evidence in monomethylhydrazine cells except at the highest concentration. The freeze-cleave technique showed that phenylhydrazine exposed erythrocytes contained Heinz bodies within two hours. The Heinz bodies formed in the central cytoplasm and migrated to the membrane. The membrane was evaginated and dimpled where Heinz bodies were near the membrane and this might represent the damage which initiates sequestration of erythrocytes by the spleen and liver. The freeze-cleave technique showed that monomethylhydrazine treated cells did not produce typical Heinz bodies, but did produce aggregates of granules arranged in ordered rows which may represent hemoglobin crystals.