Studies on Addiction and Withdrawal in Cell Cultures.
Final rept. 1 Mar 72-31 Aug 74,
OKLAHOMA STATE UNIV STILLWATER DEPT OF BIOCHEMISTRY
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After 645 days growth in the presence of slowly increasing drug concentrations and maintaining at LD50 concentrations, KB cells with increased tolerance to amphetamine, benadryl, caffeine, codeine, meprobamate, and morphine have been developed. Complete withdrawal of morphine from these cultures does not kill them therefore, it is concluded that they are not addicted. Although withdrawal symptoms were not observed in these cultures, increased tolerance and biochemical changes were observed. The effect of morphine on the cell membrane of nonneural cells gives a clue to possible widespread biological effects in all tissues whose relevancy in the addicted human needs to be determined.