Cryop Reservation of Human Granulocytes.
Annual rept. no. 5, 1 Dec 73-30 Nov 74,
CENTER FOR BLOOD RESEARCH BOSTON MASS
Pagination or Media Count:
The authors describe a method compatible with component blood banking for the harvesting and freezing of buffy coat leukocytes. It simplicity suggests that application of the cryotechnique employed will be adaptable to large numbers of cells taken in multiple units from single donors. The principle of the method is sedimentation of buffy coat leukocytes with hydroxyethyl starch HES to aggregate erythrocytes and concentrate the leukocytes, and cryopreservation by cooling the concentrate to -80C in the presence of residual HES and 5 dimenthylsulfoxide DMSO.
- Medicine and Medical Research