Cultivation of Hepatitis Virus in Tissue Culture.
Annual rept. 1 Jan-24 Dec 74,
CALIFORNIA UNIV LOS ANGELES DEPT OF MEDICINE
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The authors major efforts have been devoted to attempts at cultivation of Hepatitis B virus by using slow virus techniques. By means of co-cultivation, mixed monolayers of normal hepatocytes were co-cultivated with hepatocytes obtained from liver biopsies of patients with Hepatitis B Surface Antigen positive chronic aggressive hepatitis. The cell layers which resulted are being maintained for long periods and are also being sub-cultured for evidence of either cytopathogenic change or for evidence of multiplication of Hepatitis B Surface Antigen as determined by the concentration of antigen in serial pour offs as assayed by the radioimmunoassay. Similarly, feeder layers have been established using chronic active hepatitis liver cells as the feeder source and primary human hepatocytes as the feeder layer. Feeder layers are also being formed using other sensitive tissue culture lines including human diploid lung cells WI 38 and Hela cells.