Accession Number:

ADA002796

Title:

Genetic and Biochemical Studies of Drug Resistance in Pathogenic Bacteria.

Descriptive Note:

Final rept. 14 Aug 73-13 Aug 74,

Corporate Author:

EPISOME INST FUJIMI-MURA (JAPAN)

Personal Author(s):

Report Date:

1974-11-06

Pagination or Media Count:

16.0

Abstract:

Sixty strains of Pseudomonas aeruginosa were selected from clinical stock cultures and used as donors of drug resistance to examine the presence of R factors. They were highly resistant to one or more of six drugs including tetracyclineTC, chloramphenicolCM, streptomycin SM, sulfanilamide SA, carbenicillin CPC, and kanamycin KM. Three FP-, auxotrophic mutants of P. aeruginosa were used as recipients, which had been made resistant to either rifampin RMP or KM by in vitro mutation. Each donor was mated with each of the three recipients, and 45 strains were found to transfer their resistance to one or more recipients. All of these R factors could not be transmitted to other species belonging to the family Enterobacteriaceae. Cultures of clinical origin Providencia strains were resistant to various aminoglycoside antibiotics including lividomycin and the gentamicin C complex but sensitive to kanamycin A and a new kanamycin derivative, BB-K8. The antibacterial activity of sisomicin SS, a new aminoglycoside antibiotic active toward clinical isolates of Pseudomonas aeruginosa, was determined and compared with that of the gentamicin C complex.

Subject Categories:

  • Microbiology
  • Pharmacology

Distribution Statement:

APPROVED FOR PUBLIC RELEASE