The Physiological Bases for Microbial Barotolerance.
Annual technical rept. no. 3, 31 Dec 73-31 Dec 74,
ROCHESTER UNIV N Y DEPT OF MICROBIOLOGY
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Work during the project period focused on microbial adaptive enzyme synthesis under pressure, on the role of adenosine triphosphate supply in determining microbial barotolerance, on cell death caused by 1000 atm pressure and on growth optimization in the pressure-temperature domain. It was found that some types of adaptive enzyme synthesis were no more pressure sensitive than was protein synthesis in general. But derepression of the lac operon of Escherichia coli or the penicillinase genes of Bacillus licheniformis was more sensitive to pressure than was growth or general protein synthesis. Therefore, under pressure E. coli cells than had to adapt to lactose utilization or B. licheniformis cells that had to adapt to penicillin were more barosensitive than were previously adapted cells or cells of constitutive mutants. Thus, the pressure sensitivities of the adaptive processes compromised survival capacities under pressure.