Delineation of Immunogenic Regions by Tolerance Induction.
Annual rept. no. 1, Nov 73-30 Sep 74,
WAYNE STATE UNIV DETROIT MICH SCHOOL OF MEDICINE
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Specific suppression of the immune response to a chemically defined protein antigen is studied to determine whether portions of this antigen will suppress antibody formation and to delineate the effect in chemical and biological terms. This work is designed to increase the basic understanding of the mechanisms of immunologic tolerance, a phenomenon in which an antigen administered in a certain defined manner specifically inhibits the immune response. Immunologic tolerance appears to play a definite role in the prevention of autoimmune disease and in organ transplantation as tested in experimental animals. The antigen, oxidized ribonuclease, was chosen as a model because it is a well characterized and unfolded single peptide chain of 124 amino acids. Conditions required for an optimal immune response to a single dose of antigen are partially oxidized RNase and Freunds complete adjuvant with added mycobacteria. Unresponsiveness tolerance is best induced by giving 50 microgram of oxidized RNase in 3 daily intravenous doses starting 11 days before the immunizing dose.
- Medicine and Medical Research