Choline Transport by Neuroblastoma Cells in Tissue Culture.
Technical rept. Nov 73-Apr 74,
EDGEWOOD ARSENAL ABERDEEN PROVING GROUND MD
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Using neuroblastoma cells in tissue culture, the transport and metabolism of the acetylcholine ACh precursor, choline was examined. Acetylcholinesterase was inhibited so that any ACh formed would not be hydrolyzed enzymatically. Although the transport constant KT and capacity Vmax are similar to those previously reported, the disposition of choline which is taken up is not that expected for neural cells. Very little 3H-acetylcholine or free 3H choline is found in the cells after brief exposure to choline labeled with 3H. Instead, most of the label is found in phosphoryl-3H choline which serves as a precursor for 3H phosphatidyl choline. On the basis of these data and the effects of inhibitors of transport, it is concluded that clones of neuroblastoma cells do not possess the type of choline transport system unique to cholinergic neural tissues, although they are still useful for the study of acetylcholinesterase synthesis. Modified author abstract