Accession Number:

ADA000527

Title:

The Location of Spin Probes in Two Phase Mixed Lipid Systems,

Descriptive Note:

Corporate Author:

NATIONAL RESEARCH COUNCIL OF CANADA OTTAWA (ONTARIO) DIV OF BIOLOGICAL SCIENCES

Personal Author(s):

• Butler,K. W.
• Tattrie,N. H.
• Smith,I. C. P.

Report Date:

1974-03-21

Pagination or Media Count:

11.0

Abstract:

The authors observed electron spin resonance ESR spectra of either a cholestane or a stearic acid spin probe in liposomes formed of egg, yeast or dipalmitoyllecithins individually or in mixtures and found the liquid lines often observed with the stearic acid probe and liposomes originate with the probe free in solution the lipid-water partition coefficient of the stearic acid probe increases with increasing fluidity of the lipid the lipid-water equilibrium of the stearic acid probe is rapid even with fluid lipids liquid lines can appear in the ESR spectra at high dilutions or high spin probelipid ratios the cholestane spin probe produces spectra with no liquid lines, which in most cases represent the fluid and crystalline phases of mixed systems in proportion to their relative concentrations.

Descriptors:

• *LIPIDS
• *ELECTRON PARAMAGNETIC RESONANCE
• *STEARIC ACID
• CRYSTALS
• FLUIDS
• CHEMICAL EQUILIBRIUM

Subject Categories:

• Biochemistry

Distribution Statement:

APPROVED FOR PUBLIC RELEASE