Immunological Study of the O-Antigens of Streptomycin-Dependent Mutants of Salmonella
FOREIGN TECHNOLOGY DIV WRIGHT-PATTERSON AFB OH
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During the immunization of mice and rats with O-antigen of the streptomycin-dependent mutant 9213 of S.enteritidis, mainly IgG-antibodies were synthesized, whereas during immunization with O-antigen of the initial strain, predominantly IgM- and in an insignificant measure IgG-antibodies were synthesized. The dynamics of formation of antibodies to the O-antigen of freshly isolated streptomycin-dependent mutant 9212, the polysaccharide component of which only the end sugar tyvelose is not revealed, did not differ from the dynamics of the formation of antibodies to normal O-antigen. Lipopolysaccharide 9213, which did not contain sugar of the determinant groups, was not active in the reaction of passive skin anaphylaxis in guinea pigs. The lipopolysaccharides extracted from the mutants were toxic for mice to the same degree as the lipopolysaccharides of the initial strain.