Obtaining Bacterial Allergens by Alkaline Extraction and Research on Their Physicochemical Properties,
FOREIGN TECHNOLOGY DIV WRIGHT-PATTERSON AFB OHIO
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A common method of obtaining allergens from bacteria of the group of particularly dangerous infections and of certain representatives of enteric family is suggested as a result of the work carried out. The method consists in extraction from microbial cells of alkali-soluble components, their precipitation with acid dialysis and lyophilization. The protein part of the allergens mainly contained the fraction with a high molecular weight. Thermal treatment of certain allergens tularemic and typhoid was accompanied by the rupture of bond in the protein molecule, and separation of fractions with a lesser molecular weight some retained their specific activity.