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Influence of Neodymium-Doped Yttrium Aluminum Garnet Laser Irradiation on Cell Proliferation and Bone Morphogenetic Protein-2 Concentrations in MG-63 Osteoblast-Like Cells: An in Vitro Study

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[Technical Report, Master's Thesis]

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Objective The aim of this in vitro study was to assess the influence of neodymium-doped yttrium aluminum garnet NdYAG laser irradiation on proliferation and bone morphogenetic protein-2 BMP-2 concentrations in MG-63 osteoblast-like cells. Methods We cultured MG-63 cells in plastic 96-well plates and exposed the cultures to NdYAG laser irradiation 1064 nm. Independent variables included power 0.25 to 5.0 W and irradiation time 10 to 60 s. In test and control groups, we assessed BMP-2 protein concentrations using two methods enzyme-linked immunosorbent assay ELISA and magnetic microsphere BMP-2 assay. In addition, we assessed cell viability and proliferation using water soluble tetrazolium WST-1 and cell counting kit-8 CCK-8 assays. We evaluated outcome variables 24 and 72 h following laser exposure individual analyses and used Mann-Whitney U tests and Kruskal-Wallis tests to determine if outcomes differed across the independent variables of interest. Results Magnetic microsphere analysis failed to identify any statistically significant influence of laser energy on BMP-2 concentration irrespective of power or irradiation time. In the ELISA analysis, BMP-2 concentration was significantly higher for irradiation time of 10 versus 60 seconds p0.009 at the 24-hour assessment only. CCK-8 analysis showed that, compared with control cultures, cell proliferation was significantly higher at the 24-hour recording for cells receiving power of 0.25 W p0.0048, 1 W p0.0048, 3 W p0.0048, or 5 W p0.0048. We observed comparable effects on cell proliferation regardless of assessment time 24 or 72 h or cell proliferation assay WST-1 or CCK-8. Conclusion Under the conditions described, NdYAG laser irradiation consistently increased proliferation of MG-63 cells. However, in almost all experiments, BMP-2 protein concentrations in control and laser-treated cell cultures were not statistically different.


Subject Categories:

  • Lasers and Masers
  • Geology, Geochemistry and Mineralogy

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[A, Approved For Public Release]