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Accession Number:
AD1182876
Title:
Efficacy of Various Decontamination Methods and Sterilization on Contaminated and Inoculated Diamond-Coated Burs
Descriptive Note:
[Technical Report, Master's Thesis]
Corporate Author:
UNIFORMED SERVICES UNIV OF THE HEALTH SCIENCES BETHESDA MD
Report Date:
2020-05-27
Pagination or Media Count:
19
Abstract:
The objective of this study was to evaluate the effectiveness of various decontamination methods and subsequent sterilization on contaminated and inoculated diamond-coated burs. Diamond-coated burs and extracted human molars were sterilized with a steam sterilizer. Enamel and dentin from the extracted teeth were abraded utilizing diamond-coated burs using a high-speed handpiece. The burs were subsequently inoculated with one of the following microorganisms Enterococcus faecalis ATCC 19433, Staphylococcus aureus ATCC 6538, Pseudomonas aeruginosa ATCC 15442 or Geobacillus stearothermophilus ATCC7953. Twenty-four hours after inoculation, the burs were subjected to various cleaning treatments, sterilized, and then cultured for bacterial contamination. The number of CFUmL was determined per group. Except for the positive control group, no CFUmL or growth was found for all treatment groups and for all bacterial types. In conclusion, the contaminated and inoculated diamond-coated burs tested in this study were successfully sterilized to eliminate the tested bacteria. The use of a cleaning stone with manual cleaning or an ultrasonic cleaner resulted in the least amount of remaining tooth debris on the diamond-coated bur heads.
Distribution Statement:
[A, Approved For Public Release]
