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Mammary Tissue Host-Microbiome Interactions in Breast Cancer Development

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[Technical Report, Annual Report]

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Through this project we will determine the role of the mammary tissue microbiome in breast cancer development using16S ribosomal RNA sequencing and dual-transcriptomic sequencing. In the first two years of this project we have selected and received165 samples from the Susan G. Komen and Indiana University Simon Cancer Center Tissue Banks. We completed 16S rRNA sequencing on DNA isolated from all samples in this cohort and note a distinct microbial compositional and functional signature that is associated with breast cancer development. We are hopeful our manuscript outlining these findings will be accepted in late Fall 2021. We are currently revising this manuscript for resubmission to mSystems. We have opted to transition to conduct whole metagenome sequencing to assess genetic predisposition to mammary microbiome colonization, based on findings from our 16S microbiome analysis. Due toCOVID-19, analyses outlined in aims II and III were delayed due to Pepperdine campus being under restricted access from March 2020 to May 2021. Regardless of these delays, the project is well underway and we are confident we will complete these analyses by Summer2022. Results from this work will be key in characterizing host-microbiome cross-talk in the pathogenesis of breast tumor development.


Subject Categories:

  • Medicine and Medical Research

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[A, Approved For Public Release]