Accession Number:

AD1155537

Title:

Understanding the 'Mission Versatility' of Membrane Proteins via Nanoscopic Imaging

Descriptive Note:

[Technical Report, Final Report]

Corporate Author:

UNIVERSITY OF ILLINOIS

Personal Author(s):

• Chen, Qian

Report Date:

2021-09-30

Pagination or Media Count:

10

Abstract:

The goal of this AFOSR Young Investigator Program grant is to investigate the nanoscale structural and functional dynamics of membrane proteins in their native liquid environment using liquid phase transmission electron microscopy TEM. Over the project time period, we optimized the self-assembly and purification of nanodiscsmembrane proteins in a single-particle form amenable to TEMobservationcontaining the protein cytochrome p450 2J2 CYP-2J2 and then developed a graphene liquid sandwich to encapsulate CYP- 2J2 nanodiscs in their native buffer conditions for imaging.

Descriptors:

Subject Categories:

• Biochemistry
• Atomic and Molecular Physics and Spectroscopy

Distribution Statement:

[A, Approved For Public Release]