Assessing Pollinator Communities via Environmental DNA (eDNA) Metacommunity Assay
[Technical Report, Final Report]
ILLINOIS NATURAL HISTORY SURVEY CHAMPAIGN IL
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Pollinators provide essential ecosystem services and are critical components of resilient ecosystems. Yet, pollinators are suffering staggering global declines leading to increased listing under the Endangered Species Act. This presents challenges for DoD installations. The technical objectives of this project were to 1 develop and validate broad-spectrum pollinator eDNA metabarcoding primers, 2 test the efficacy of multiple capture methods to obtain Next Generation Sequencing-ready pollinator eDNA from flowers, and 3 validate eDNA sampling and microfluidic metabarcoding methods in controlled and field experiments with mixed plantpollinator communities.
- Genetic Engineering and Molecular Biology