Accession Number:

AD1135346

Title:

Assessing Pollinator Communities via Environmental DNA (eDNA) Metacommunity Assay

Descriptive Note:

[Technical Report, Final Report]

Corporate Author:

ILLINOIS NATURAL HISTORY SURVEY CHAMPAIGN IL

Report Date:

2020-04-06

Pagination or Media Count:

72

Abstract:

Pollinators provide essential ecosystem services and are critical components of resilient ecosystems. Yet, pollinators are suffering staggering global declines leading to increased listing under the Endangered Species Act. This presents challenges for DoD installations. The technical objectives of this project were to 1 develop and validate broad-spectrum pollinator eDNA metabarcoding primers, 2 test the efficacy of multiple capture methods to obtain Next Generation Sequencing-ready pollinator eDNA from flowers, and 3 validate eDNA sampling and microfluidic metabarcoding methods in controlled and field experiments with mixed plantpollinator communities.

Subject Categories:

  • Ecology
  • Genetic Engineering and Molecular Biology

Distribution Statement:

[A, Approved For Public Release]